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RNA-Seq with Bioconductor in R

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$12

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Course Features

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Duration

4 hours

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Delivery Method

Online

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Available on

Limited Access

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Accessibility

Mobile, Desktop, Laptop

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Language

English

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Subtitles

English

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Level

Intermediate

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Teaching Type

Self Paced

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Video Content

4 hours

Course Description

RNA-Seq is a next-generation sequencing technique which identifies genes or pathways that are responsible for certain diseases and conditions. It's exciting. As high-throughput sequencing data becomes more affordable and easier to access, the ability to analyze it is becoming a valuable skill. You will learn about the RNA sequencing process and how to identify genes or biological processes that might be relevant for you. This course will give you a brief overview of the RNA sequencing process, with a special focus on differential expression (DE). The course will start with gene counts. The course will then discuss how to prepare data for DE analysis. The DESeq2 package is used to model the count data using a negative binary model, and test for differentially expressed genes. You can visualize the results with heatmaps and volcano plots. You can save the genes that are differentially expressed.

Course Overview

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Virtual Labs

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International Faculty

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Post Course Interactions

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Hands-On Training,Instructor-Moderated Discussions

Skills You Will Gain

Prerequisites/Requirements

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Introduction to Bioconductor in R

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Introduction to Data Visualization with ggplot2

What You Will Learn

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Use RNA-Seq differential expression analysis to identify genes likely to be important for different diseases or conditions

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Starting with the counts for each gene, the course will cover how to prepare data for DE analysis, assess the quality of the count data, and identify outliers and detect major sources of variation in the data

Course Instructors

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Mary Piper

Bioinformatics Consultant and Trainer

Mary Piper serves dual roles as research analyst and bioinformatics trainer in the Department of Biostatistics at the Harvard T.H. Chan School of Public Health. However, her primary role is the devel...

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Course Cover

$12

$6

49% OFF

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